生物脫硫技術(shù)應用的主要細菌是氧化亞鐵硫桿菌，該菌種的生活條件是怎樣的呢？我們又該如何提高氧化亞鐵硫桿菌的反應效率呢？我們一起來看一下吧。
Thiobacillus ferrooxidans is the main bacteria used in biological desulfurization technology. What is the living condition of the strain? How can we improve the reaction efficiency of Thiobacillus ferrooxidans? Let's have a look.
氧化亞鐵硫桿菌具有嗜酸、適于中溫環(huán)境等特性，廣泛存在于酸性礦山水及含鐵或硫的酸性環(huán)境中，是一種重要的浸礦微生物。近年來在生物脫硫技術(shù)中得到了廣泛的應用。那么氧化亞鐵硫桿菌的培養(yǎng)要注意什么呢?
Thiobacillus ferrooxidans is an important leaching microorganism, which is acidophilic and suitable for medium temperature environment. It widely exists in acidic mine water and acidic environment containing iron or sulfur. In recent years, it has been widely used in biological desulfurization technology. So what should we pay attention to in the cultivation of Thiobacillus ferrooxidans?
據(jù)研究表明：1.氧化亞鐵硫桿菌適合在初始pH為2.0時生長, 此條件下, 細菌很快適應環(huán)境, 可以正常吸收營養(yǎng)物質(zhì), 合成新的原生質(zhì), 形成一定量細菌所必需的酶；
According to the research, 1. Thiobacillus ferrooxidans is suitable to grow at the initial pH of 2.0. Under this condition, the bacteria quickly adapt to the environment, can normally absorb nutrients, synthesize new protoplasts, and form a certain amount of enzymes necessary for bacteria.
2.氧化亞鐵硫桿菌的佳培養(yǎng)溫度是30℃.在這兩個溫度范圍內(nèi), 酶活性高, 細胞的生物化學反應速度和生長速度較快; 但微生物機體的重要組成, 如核酸、蛋白質(zhì)等對溫度較敏感, 隨著溫度的升高可遭受不可逆的破壞.
2. The optimum culture temperature of Thiobacillus ferrooxidans is 30 C. Within these two temperature ranges, the enzyme activity is high, the biochemical reaction rate and growth rate of cells are fast; but the important components of microbial organisms, such as nucleic acid and protein, are sensitive to temperature and can be irreversibly destroyed with the increase of temperature.
3.當接種適量增大時, 培養(yǎng)基中的初始菌數(shù)增加, 氧化亞鐵硫桿菌快速繁殖.但由于培養(yǎng)基中的營養(yǎng)物質(zhì)有限, 加入過多的菌液影響細菌的生長繁殖.表明培養(yǎng)氧化亞鐵硫桿菌的佳接種量為10%.
3. When the inoculation amount increased, the initial number of bacteria in the culture medium increased, and Thiobacillus ferrooxidans multiplied rapidly. However, due to the limited nutrients in the culture medium, excessive bacterial liquid was added to affect the growth and reproduction of bacteria. It showed that the optimal inoculation amount of Thiobacillus ferrooxidans was 10%.
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2019-03-13
